2-硝基苯基-β-D-吡喃半乳糖苷, 99%，非動物源
Product Name： 2-Nitrophenyl β-D-galactopyranoside
別名:2-硝基苯基-β-D-吡喃半乳糖苷(ONPG) ;鄰硝基苯-β-D-吡喃半乳糖苷
CAS號:369-07-3
分子式:C12H15O8N
分子量:301.25
介紹： 溶于水、甲醇，水中溶解度可達15mg/ml。有吸濕性。
用途： 用于檢測β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的顯色底物。經β-半乳糖苷酶催化后可產生黃色產物，在410－420nm波長可以檢測吸光度。
熔點： 195℃
特定比重： 1.599
比旋光度： -68.8 ° (C=1, H2O)
貯存： -20℃充氬保存
生化和生理學機理： β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
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